Solid Approach to cfDNA Purification REQUEST A SAMPLE
Predispensed solid reagents in individual tubes designed for a specific volume of sample.
Convenient - Minimal sample handling and pipetting.
Solid Phase Reagents - Accurate weight ensures optimal cfDNA recovery
Minimal sample dilution - no buffers added to plasma sample
PACs - Protease Activated Capsules for Pretreatment of Samples REQUEST A SAMPLE
Dissolvable Packets containing buffer and enzyme mix - just add sample
Stable at room temperature
Preweighed packets for convenience and accuracy
N52 - Magnetic Separator for Microtiter and Manual Formats from 200uL to 250mL SEE ALL THE MAGNETS
MagPen for large single sample purification
6 Pillar, 6 Pillar ExtraDeep and 24 Pillar magnet/sleeve for 24 well plates.
8-Channel, 12-Channel, and 96 Pillar magnet/sleeves for 96 well plates.
Strongest available permanent magnets
Stand configurations from 30ul to 50ml
Pillars in 24 and 96 well formats for minimal handling / semi and full automation
Magnet/sleeve combination eliminates the need for aspiration and resuspension.
Solid Reagents Optimal for Larger Samples - Minimal Dilution REQUEST A SAMPLE
Cell Free DNA is typically present at 5 to 10 ng per milliliter of plasma
The implication is about 2,000 genome equivalents per milliliter
20,000 genomes is about 60 ng which requires about 10 milliliters of plasma - about two tubes of whole blood required
Proteolytic digestion with solid predispensed Protease PACs combined with solid predispensed powder binding reagents minimizes
sample dilution which aids in cfDNA capture.
Note: Once the protease packet (PACs) is dissolved in the plasma, the cfDNA is stable at room temperature for greater than 2 weeks
Note: For detection at 0.1%, 10,000 genomes will yield 10 events.
Magnetic particle separation provides a simple and efficient
method for detection and purification of nucleic acids, proteins and cells.
NeoGeneStar is focused on the manufacture of magnetic particles and kits for
magnetic molecular purification. Our state-of-art facility for manufacturing of
the magnetic particles and molecular purification kits is a cGMP compliant manufacturing
NeoGeneStar is devoted to the advancement of the methods for the purification of circulating cell-free DNA (cfDNA) in the fields of non-invasive prenatal testing (NIPT) and cancer diagnostics. NeoGeneStar circulating DNA kits utilize proprietary superparamagnetic beads and patent-pending solid lysis reagens designed for isolation of circulating cell-free DNA in plasma, serum or urine and CSF, especially for larger sample volume.
The NeoGeneStar cfDNA purification kits are designed to fit the need of customers. Off-the-shelf kits are available for sample volumes of 0.1ml to 20ml and can be processed in typically available centrifuge tubes such as micro-centrifuge tube, 15ml and 50ml centrifuge tube. Our larger volume kits are especially suitable for purification of cfDNA from about 4-20ml plasma or urine.
NeoGeneStar offer innovative handheld magnets amenable for automated and manual processing. we've developed a new line of magnet / sleeve products which eliminate the aspiration and resuspension steps in magnetic particle purification by moving the particles on a plastic sleeve covering the magnets. We have arrays for most microtiter plate formats, 6, 24 & 96 wells as well as specialized extra long magnets for tubes and tissue culture, these magnets can be used for as little as 30ul to as much as 250 ml of biological fluids.